K-SUFRG | Sucrose/D-Fructose/D-Glucose Assay Kit

Product code: K-SUFRG

The Sucrose/D-Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.

Description

UV-method for the determination of Sucrose, D-Fructose and D-Glucose in foodstuffs, beverages and other materials

 

Principle:

(β-fructosidase)
(1) Sucrose + H2O → D-glucose + D-fructose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(5) F-6-P   →     G-6-P

 

Kit size:   * 100 assays of each

 

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

 

Method:                             Spectrophotometric at 340 nm
Reaction time:                 ~ 20 min
Detection limit:                1.38 mg/L

Application examples:
Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, dairy products, candies, desserts, confectionery, sweets, ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage), condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics, pharmaceuticals, paper and other materials

Method recognition:
Methods based on this principle have been accepted by NF, EN, NEN, DIN, GOST, IFU, AIJN, MEBAK and IOCCC

DATA SHEET: K-SUFRG_DATA

MEGA CALC: K-SUFRG_CALC

 

Advantages:

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Stabilised D-glucose / D-fructose standard solution included
  • Extended cofactors stability