K-RSTAR | Resistant Starch Assay Kit

Product code: K-RSTAR

The Resistant Starch Test kit for the measurement and analysis of resistant starch in plant materials and starch samples.

Description

Colourimetric method for the determination of Resistant Starch in cereal products and feeds

Principle:
(α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose + maltose (trace)

(2) Aqueous ethanol wash + centrifugation to remove D-glucose + maltose

(3) Dissolution of resistant starch pellet in KOH and neutralisation

(α-amylase + amyloglucosidase)

(4) Dissolved resistant starch + H2O → D-glucose

(glucose oxidase)

(5) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)

(6) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → quinoneimine + 4H2O

Kit size:                                100 assays
Method:                              Spectrophotometric at 510 nm
Reaction time:                  ~ 120 min (plus overnight incubation)
Detection limit:                 2-100% of sample weight
Application examples:   Plant materials, starch samples and other materials

Method recognition:
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX (Type II Method)

Data Sheet: K-RSTAR_DATA

Mega Calc: K-RSTAR_CALC