K-LACTUL | Lactulose Assay Kit

Product code: K-LACTUL

The Lactulose Assay Kit is suitable for the specific, rapid and sensitive measurement and analysis of lactulose in milk and milk-based samples. Reagents included in this kit may also be prepared for use in the procedure described by ISO Method 11285:2004.

Description

UV-method for the determination of Lactulose in milk and foodstuffs containing dairy products. 

Principle:
(β-galactosidase)
(1) Lactulose + H2O → D-galactose + D-fructose

(glucose oxidase + catalase + H2O2)
(2) D-Glucose + H2O + O2 → D-gluconic acid + H2O2

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(4) F-6-P    →     G-6-P

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(gluconate-6-phosphate dehydrogenase)
(6) Gluconate-6-phosphate + NADP+ → ribulose-5-phosphate + NADPH + CO2 + H+

Kit size:                             50 assays
Method:                           Spectrophotometric at 340 nm
Reaction time:               ~ 120 min
Detection limit:             4.8 mg/L

 

Application examples:
Milk, dairy products and foods containing milk

 

Method recognition:    Novel method

 

Data Sheet:

K-LACTUL_DATA