K-LACGAR | Lactose/ D-Galactose Assay Kit (Rapid)

Product code: K-LACGAR

The Lactose/Galactose (Rapid) test kit is used for the rapid test of lactose, D-galactose and L-arabinose in food and plant products. Galactose dehydrogenase can be used the measurement and analysis of both D-galactose and L-arabinose. Suitable for the analysis of lactose in “low-lactose” or “lactose-free” samples which contain high levels of monosaccharides. The reagents provided in this kit are also suitable for use with AOAC method 2006.06 – Lactose in milk.

Description

UV-method for the determination of Lactose and D-Galactose in foodstuffs, beverages and other materials

Principle:
(β-galactosidase)
(1) Lactose + H2O → D-galactose + D-glucose

(galactose mutarotase)
(2) α-D-Galactose ↔ β-D-galactose

(β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Kit size:    115 assays

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave

Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 15 min
Detection limit:                 2.96 mg/L (lactose)

 

Application examples:
Milk, dairy products (e.g. cream, milk / whey powder, cheese, condensed milk and yogurt), foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), food additives, feed, cosmetics, pharmaceuticals and other materials
(e.g. biological cultures, samples, etc.)

 

Method recognition:
Methods based on this principle have been accepted by AOAC 2006.06, NBN, DIN, GOST and IDF

 

Data Sheet:

K-LACGAR_DATA