K-LMALAF | L-Malic Acid Assay Kit (Analyser Format)

Product code: K-LMALAF

The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Description

Analyser format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size:                               245.5 mL of prepared reagent (R1 + R2)
Method:                             Spectrophotometric at 340 nm
Reaction time:                 ~ 3 min
Detection limit:                20 mg/L (recommended assay)

 

Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.)

 

Method recognition:
Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

 

Data sheet:

K-LMALAF_DATA