K-LARGE | L-Arginine/Urea/Ammonia Assay Kit

Product code: K-LARGE

The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine. Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Description

UV-method for the determination of L-Arginine, Urea and Ammonia in grape juice, must and wine

Principle:

(arginase)
(1) L-Arginine + H2O → urea + ornithine

(urease)
(2) Urea + H2O → 2 NH3 + CO2

(microbial glutamate dehydrogenase)
(3) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP+ +
H2O

Kit size:  50 assays

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave

Spectrophotometer (D-MQWAVE).

 

Method:                                 Spectrophotometric at 340 nm
Reaction time:                   ~ 20 min [ammonia (2 min), urea (6 min), L-arginine (7 min)

Detection limit:                  0.07 mg/L (ammonia)
0.13 mg/L (urea)
0.37 mg/L (L-arginine)

 

Application examples:
Grape juice, wine must, wine and other materials (e.g. biological cultures, samples, etc.)

 

Method recognition:    improved method

 

Data Sheet:

K-LARGE_DATA