K-ARGA | L-Arabinose/D-Galactose Assay Kit

Product code: K-ARGA

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Description

UV-method for the determination of L-Arabinose and D-Galactose
in hydrolysed plant products

Principle:
                                      (galactose mutarotase)
(1) α-L-Arabinose / α-D-galactose ↔ β-L-arabinose / β-D-galactose

(β-galactose dehydrogenase)
(2) β-L-Arabinose + NAD+ → L-arabinonic acid + NADH + H+

(β-galactose dehydrogenase)
(3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Kit size:                            * 115 assays (manual) / 1150 (microplate)
/ 1150 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 12 min (L-arabinose)
~ 6 min (D-galactose)
Detection limit:                 0.58 mg/L (L-arabinose)
0.69 mg/L (D-galactose)
Application examples:
Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan,
arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods
containing milk (e.g. dietetic foods, bakery products, baby food,
chocolate, sweets and ice-cream), food additives (e.g. sweeteners),
cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:       Novel method

SDS: K-ARGA