K-AVCHO | Available Carbohydrates Kit (100 Assays)

Product code: K-AVCHO

The Available Carbohydrates 100 ASSAYS test kit is used for the measurement of available carbohydrates (total digestible starch plus maltodextrins, sucrose, D-glucose, D-fructose and the glucose component of lactose) in cereal products, foodstuffs, feeds and other materials; measured as D-glucose and D-fructose.

Description

Available Carbohydrates Kit (100 Assays)

Available Carbohydrates Assay Kit

The Available Carbohydrates Assay Kit method is suitable for the measurement of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).

* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption by the human small intestine.

Principle:
                       (PAA + AMG, pH 6.0, 37°C, 4 h)
(1) Digestible starch + H2O → D-glucose

                (sucrase)
(2) Sucrose → D-glucose + D-fructose

            (β-galactosidase)
(3) Lactose → D-galactose + D-glucose 

                                 (HK)
(4) D-Glucose + ATP → G-6-P + ADP

                                  (HK)
(5) D-Fructose + ATP → F-6-P + ADP

                          (G-6-P DH)
(6) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

             (PGI)
(7) F-6-P ↔ G-6-P

                          (GalDH/GalM)
(8) β-D-Galactose + NADP+ ↔ D-galactonate + NADPH + H+

Kit size:                               100 assays
Method:                               Hydrolysis
Total assay time:                ~ 5 h
Detection limit:                   2-100% of sample weight
Application examples: 
Food ingredients, food products and other materials

Data Sheet: Click Here
Mega-Calc: Click Here