K-GCROL | Glycerol Assay Kit
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Description
UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials
Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP
(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate
(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+
Kit size: * 70 assays (manual) / 700 (microplate)
* The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK
