K-FRUCHK | Fructan Assay Kit (Hexokinase format)

Product code: K-FRUCHK

The Fructan HK test kit is suitable for the specific measurement and analysis of all fructooligosaccharides (reducing and non-reducing) and of fructan polysaccharide.

Description

UV-method for the determination of Fructan in foodstuffs, beverages and other materials

Principle:
(sucrase, pH 6.5, 40°C)
(1) Sucrose + H2O → D-glucose + D-fructose

(Maltase, pH 6.5, 40°C)
(2) maltose + maltotriose → D-glucose

(hexokinase + phosphoglucose isomerase)
(3) D-Glucose + D-fructose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(exo-inulinase + endo-inulinase, pH 4.5, 40°C)
(5) Fructan + H2O        →        D-glucose + D-fructose

Kit size:                              50 assays
Method:                              Spectrophotometric at 340 nm
Total assay time:               ~ 30 min
Detection limit:                  1-100% of sample weight

 

Application examples:
Flours, plant materials (e.g. onion), food products and other materials

 

Method recognition:
This method is a modification of AOAC Method 999.03 and AACC Method 32-32.01

Data Sheet:

K-FRUCHK_DATA