K-ETOH | Ethanol Assay Kit

Product code: K-ETOH

The Ethanol test kit is a simple, reliable and accurate method for the measurement and analysis of ethanol in beverages and foodstuffs. Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Description

UV-method for the determination of Ethanol in foodstuffs, beverages, and other materials

Principle:
(alcohol dehydrogenase)
(1) Ethanol + NAD+ → acetaldehyde + NADH + H+

(aldehyde dehydrogenase)
(2) Acetaldehyde + NAD+ + H2O → acetic acid + NADH + H+

Kit size:     * 60 assays (manual) / 600 (microplate)/ 600 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                             Spectrophotometric at 340 nm
Reaction time:                   ~ 5 min
Detection limit:                  0.093 mg/L

 

Application examples:
Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic/ non-alcoholic beverages, pickles, fruit and fruit juice, chocolate products, vinegar, jam, bread and bakery products, honey, soy sauce, dairy products, cosmetics, pharmaceuticals and other materials(e.g. biological cultures, samples, etc.)

 

Method recognition:
Methods based on this principle have been accepted by IFU, EBC, MEBAK and ASBC

 

Data Sheet:

K-ETOH_DATA