K-SORB | Sorbitol/Xylitol Assay Kit

Product code: K-SORB

The D-Sorbitol/Xylitol test kit is suitable for the measurement and analysis of D-sorbitol and/or xylitol in food products.

Suitable for manual, auto-analyser and microplate formats.

Description

Colourimetric method for the determination of D-Sorbitol and Xylitol in foodstuffs and wine

Principle:
(sorbitol dehydrogenase)

(1) D-Sorbitol + NAD+ ↔ D-fructose + NADH + H+

(sorbitol dehydrogenase)
(2) Xylitol + NAD+ ↔ D-xylulose + NADH + H+

(diaphorase)
(3) INT + NADH + H+ → NAD+ + INT-formazan

Kit size:   * 58 assays (manual) / 580 (microplate / 700 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                               Spectrophotometric at 492 nm
Reaction time:                  ~ 15 min
Detection limit:                 0.20 mg/L

Application examples:
Diabetic foods (e.g. honey, jam and chocolate), dietetic foods, chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets, bakery products (e.g. desserts), marzipan, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.)

Method recognition:
Methods based on this principle have been accepted by IFU and AIJN

Data Sheet: K-SORB_DATA

Mega-Calc: K-SORB_CALC