K-MANGL | D-Mannose/D-Fructose/D-Glucose Assay kit

Product code: K-MANGL

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

Description

UV-method for the determination of D-Mannose, D-Fructose
and D-Glucose in foodstuffs, yeast cell preparations and
other materials

Principle:
(hexokinase)
(1) D-Mannose / D-fructose / D-glucose + ATP →
M-6-P / F-6-P / G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphomannose isomerase) (phosphoglucose isomerase)
(3)  M-6-P              ↔              F-6-P              ↔              G-6-P

Kit size:                             * 55 assays

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 30 min
Detection limit:                 0.7 mg/L
Application examples:
Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other
materials (e.g. biological cultures, samples, etc.)
Method recognition:   Novel method

SDS: K-MANGL