K-URONIC | D-Glucuronic/D-Galacturonic Acid Assay Kit

Product code: K-URONIC

The D-Glucuronic/D-Galacturonic test kit is a simple, reliable and accurate method for the measurement and analysis of D-hexuronic acids (specifically
D-glucuronic acid and D-galacturonic acid) in plant extracts, culture media/supernatants and other materials.

Description

UV-method for the determination of D-Glucuronic Acid or
D-Galacturonic Acid in hydrolysates of plant material and
polysaccharides and other materials

Principle:
(Uronate dehydrogenase; UDH)
(1) D-Glucuronic acid + NAD+ + H2O → D-glucarate + NADH + H+

(Uronate dehydrogenase; UDH)
(2) D-Galacturonic acid + NAD+ + H2O → D-galactarate + NADH + H+

Kit size:                             * 100 assays (manual) / 1000 (microplate)
/ 1000 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                             Spectrophotometric at 340 nm
Reaction time:                  ~ 10 min at 25°C or ~ 5 min at 37°C
Detection limit:                 ~ 17 mg/L
Application examples:
Hydrolysates of plant material and polysaccharides and other
materials
Method recognition:   Novel method