K-GLUHK-110A | D-Glucose Assay Kit (Hexokinase Format) 110A

Product code: K-GLUHK-110A

The D-Glucose HK (Regular) test kit is a high purity reagent for the measurement and analysis of D-glucose in plant and food products. Can be used in combination with other Megazyme's products that require glucose determination.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

Description

UV-method for the determination of D-Glucose in foodstuffs,
beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                            (K-GLUHK-110A)
110 assays (manual) / 1100 (microplate)
/ 1000 (auto-analyser) or
(K-GLUHK-220A)
220 assays (manual) / 2200 (microplate)
/ 2000 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 5 min
Detection limit:                  0.66 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery
products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals
(e.g. infusions), feed, paper (and cardboard) and other materials (e.g.
biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN and MEBAK