K-GAMINE | D-Glucosamine Assay Kit (plus Glucosamine-Sulphate and -Acetate)

Product code: K-GAMINE

The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products.

Description

UV-method for the determination of D-Glucosamine,
D-Glucosamine sulphate and N-Acetyl Glucosamine in food
supplements, foodstuffs, beverages and other materials

Principle:
(desulphation)
(1) Glucosamine sulphate → D-glucosamine + sulphate

(deacetylation)
(2) N-Acetyl Glucosamine → D-glucosamine + acetate

(hexokinase)
(3) D-Glucosamine + ATP → D-glucosamine-6-P + ATP

(glucosamine 6-phosphate deaminase)
(4) D-Glucosamine-6-P + H2O → D-fructose-6-P + NH4+

(phosphoglucose isomerase)
(5)  F-6-P             →            G-6-P

(glucose-6-phosphate dehydrogenase)
(6) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size:                             * 50 assays (manual) / 500 (microplate)
/ 500 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                             Spectrophotometric at 340 nm
Reaction time:                   ~ 8 min
Detection limit:                 1.33 mg/L
Application examples:
Food supplements, food products and beverages
Method recognition:     Novel method