K-GAMINE | D-Glucosamine Assay Kit (plus Glucosamine-Sulphate and -Acetate)
The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products.
Description
UV-method for the determination of D-Glucosamine,
D-Glucosamine sulphate and N-Acetyl Glucosamine in food
supplements, foodstuffs, beverages and other materials
Principle:
(desulphation)
(1) Glucosamine sulphate → D-glucosamine + sulphate
(deacetylation)
(2) N-Acetyl Glucosamine → D-glucosamine + acetate
(hexokinase)
(3) D-Glucosamine + ATP → D-glucosamine-6-P + ATP
(glucosamine 6-phosphate deaminase)
(4) D-Glucosamine-6-P + H2O → D-fructose-6-P + NH4+
(phosphoglucose isomerase)
(5) F-6-P → G-6-P
(glucose-6-phosphate dehydrogenase)
(6) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
Kit size: * 50 assays (manual) / 500 (microplate)
/ 500 (auto-analyser)
* The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).
Method: Spectrophotometric at 340 nm
Reaction time: ~ 8 min
Detection limit: 1.33 mg/L
Application examples:
Food supplements, food products and beverages
Method recognition: Novel method