K-GATE | D-Gluconate Acid/D-Glucono-δ-lactone Assay Kit

Product code: K-GATE

The D-Gluconic Acid/D-Glucono-δ-lactone test kit is suitable for the specific measurement and analysis of D-gluconic acid/D-gluconolactone in foods and beverages.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Description

UV-method for the determination of D-Gluconic Acid and
D-Glucono-δ-lactone in foodstuffs, beverages and other
materials

Principle:
(gluconate kinase)
(1) D-Gluconate + ATP → gluconate-6-phosphate + ADP

(gluconate-6-phosphate dehydrogenase)
(2) Gluconate-6-phosphate + NADP+ → ribulose-5-phosphate +
NADPH + CO2 + H+

(pH 11)
(3) D-Glucono-δ-lactone + H2O → D-gluconate

Kit size:    60 assays (manual) / 600 (microplate)
/ 600 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 6 min
Detection limit:                 0.792 mg/L
Application examples:
Wine, meat, processed meat (e.g. additives), fruit juice, dairy products,
pharmaceuticals, paper and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Methods based on this principle have been accepted by ISO,
DIN and GOST