K-FRUGL | D-Fructose/ D-Glucose Kit (110 Assays)
D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products. Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of D-Fructose and D-Glucose in foodstuffs, beverages and other materials
(1) D-Glucose + ATP → G-6-P + ADP
(2) D-Fructose + ATP → F-6-P + ADP
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(4) F-6-P ↔ G-6-P
Kit size: * 110 assays (manual) / 1100 (microplate) / 1100 (auto-analyser)
* The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Method: Spectrophotometric at 340 nm
Reaction time: ~ 13 min
Detection limit: 0.66 mg/L
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.)
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC