K-FRUGL | D-Fructose/ D-Glucose Kit (110 Assays)

Product code: K-FRUGL

D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products. Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Description

UV-method for the determination of D-Fructose and D-Glucose in foodstuffs, beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(4) F-6-P             ↔             G-6-P

Kit size:    110 assays (manual) / 1100 (microplate) / 1100 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave

Spectrophotometer (D-MQWAVE).

 

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 13 min
Detection limit:                 0.66 mg/L

 

Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.)

 

Method recognition:
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

 

Data Sheet:

K-FRUGL_DATA