K-FRGLQR | D-Fructose/D-Glucose Assay Kit (Liquid Ready Reagents)

Product code: K-FRGLQR

The D-Fructose/D-Glucose (Liquid Ready Reagents) test kit is a rapid, reliable and accurate method for the specific measurement and analysis of D-fructose and D-glucose in wine, beverages, foodstuffs and other materials. Supplied as a "ready to use" liquid stable formulation that is suitable for auto-analyser and microplate formats.

Description

UV-method suitable for auto-analyser and microplate formats for
the determination of D-Fructose and D-Glucose in foodstuffs,
beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(4) F-6-P           ↔           G-6-P

Kit size:                           1100 assays (microplate) / 1100 (auto-analyser)
Method:                           Spectrophotometric at 340 nm
Reaction time:                 ~ 13 min
Detection limit:                133 mg/L (recommended format)
Application examples:
Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods,
bread, bakery products, candies, desserts, confectionery, ice-cream,
fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals,
paper and other materials (e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC