K-FRGLMQ | The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format)

Product code: K-FRGLMQ

The D-Fructose and D-Glucose MegaQuant™ Format test kit is suitable for the measurement and analysis of D-fructose and D-glucose in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm).

Description

Simple colourimetric method for the determination of
D-Fructose and D-Glucose in foodstuffs, beverages and
other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

(phosphoglucose isomerase)
(3) F-6-P            ↔            G-6-P

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(diaphorase)
(5) INT + NADPH + H+ → NADP+ + INT-formazan

Kit size:                           60 assays
Method:                           Spectrophotometric at 505 nm
Reaction time:                 ~ 5 min
Detection limit:               23.2 mg/L
Application examples:
Grape juice / must, wine, beer, fruit juices, soft drinks, milk, jam, honey,
dietetic foods, bread, bakery products, candies, desserts, confectionery,
ice-cream, fruit and vegetables, condiments, tobacco, cosmetics,
pharmaceuticals, paper and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:      Novel method