K-AMYL | Amylose/Amylopectin Assay Kit

Product code: K-AMYL

The Amylose/Amylopectin test kit is suitable for the measurement and analysis of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure.

Description

Colourimetric method for the determination of Amylose and Amylopectin in cereals, food and feed.


Principle:
(Con A)
(1) Soluble starch (amylose + amylopectin) →amylose + amylopectin-Con A (soluble) (precipitate)

(α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H2O → D-glucose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → quinoneimine + H2O

(α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H2O → D-glucose

Kit size:                             100 assays
Method:                           Spectrophotometric at 510 nm
Total assay time:           ~ 120 min
Detection limit:             Amylose 5-95% of total starch content
Application examples:
Cereal starches, flours, pure starches and foods
Method recognition:         Novel method

Data Sheet:

K-AMYL_DATA