K-AMIAR | Ammonia Assay Kit (Rapid)
Ammonia Assay Kit, for the rapid measurement and analysis of ammonia in all samples, including grape juice and wine (and other foods/beverages).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
UV-method for the determination of Ammonia in foodstuffs, beverages and other materials
Principle: (microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH4+ → L-glutamic acid + NADP++ H2O
Kit size: * 96 assays (manual) / 960 (microplate)/ 960 (auto-analyser)
* The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.07 mg/L
Grape juice, wine, fruit juices, soft drinks, dairy products (e.g. milk), dietetic food, soy sauce, eggs and egg products, cheese, meat, processed meat, seafood, bakery products (and baking agents), fertilisers, pharmaceuticals, tobacco, cosmetics, water, Kjeldahl analysis, paper (and cardboard), water and other materials (e.g. biological cultures, samples, etc.)
Methods based on this principle have been accepted by MEBAK