K-ACETRM | Acetic Acid Assay Kit (Rapid Manual Format)

Product code: K-ACETRM

The Acetic Acid (Acetate Kinase Manual Format) test kit is suitable for the measurement and analysis of acetic acid in food and beverages.

This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials.

Description

Manual format UV-method for the determination of Acetic Acid in foodstuffs, beverages and other materials

Principle:
                         (acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP

(phosphotransacetylase)
(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi

(pyruvate kinase)
(3) ADP + PEP → ATP + pyruvate

(D-lactate dehydrogenase)
(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+

Kit size:                            72 assays (manual) / 720 (microplate)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE).

Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 4 min
Detection limit:                 0.254 mg/L
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables,
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    Improved method