K-YBGL | β-Glucan Assay Kit (Yeast & Mushroom)

Product code: K-YBGL

β-Glucan (Yeast & Mushroom) Assay Kit is suitable for the measurement and analysis of 1,3:1,6-β-glucan and α-glucan in yeast and mushroom  preparations.

New, improved procedure.

Several acid and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H2SO4. For most samples, similar β-glucan values were obtained with both the optimized HCl and H2SO4 procedures. However, in the case of certain samples, specifically Ganoderma lucidum and Poria cocus, the H2SO4 procedure resulted in significantly higher values.

McCleary, B.V. & Draga, A. (2016). Measurement of β-Glucan in Mushrooms and Mycelial Products. Journal of AOAC International, Vol. 99, No. 2.

The kit components have not been altered from previous lots.

Description

Colourimetric method for the determination of Yeast and Mushroom β-Glucan in yeast, mushroom, foodstuffs and other materials

Principle:
(12 M H2SO4, 0°C, 2 h)

(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O → soluble glucan

(1 M H2SO4, 100°C, 2 h)

(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)

(exo-1,3-β-glucanase + β-glucosidase)

(3) Laminarisaccharides + H2O → D-glucose

(glucose oxidase)

(4) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)

(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → quinoneimine + 4H2O

(amyloglucosidase)

(6) α-Glucan + H2O    →   D-glucose

Kit size:                                100 assays
Method:                              Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                1-100% of sample weight

Application examples:
Yeast preparations, mushroom preparations and other materials

Method recognition:       Novel method

Data Sheet: Click Here
SDS:
Click Here
Mega-Calc: Click Here