K-BETA3 | β-Amylase Assay (Betamyl-3)

Product code: K-BETA3

The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.

Description

Colourimetric method for the determination of β-Amylase in cereal grains, malt, food, beverages and fermentation products

Principle:
                          (β-amylase)
(1) G3-β-PNP + H2O → G2 + G-β-PNP

(β-glucosidase)
(2) G-β-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) p-Nitrophenol → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:                            100 / 200 assays
Method:                            Spectrophotometric at 400 nm
Reaction time:                  ~ 10 min
Detection limit:                 0.05 U/mL of sample solution
Application examples:
Cereal flours, malts and other materials

Method recognition:
Modification of RACI (Standard Method)

Data Sheet:

K-BETA3_DATA