K-CERA | Ceralpha: α-Amylase Assay Kit (100 Assays)

Product code: K-CERA

The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).


Colourimetric method for the determination of α-Amylase in foodstuffs, feed and fermentation products

(1) Benzylidene-G7-α-PNP + H2O → Benzylidene-GX + G(7-X)-α-PNP

(thermostable α-glucosidase)
(2) G(7-X)-α-PNP + H2O → D-glucose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:                            100 / 200 assays
Method:                            Spectrophotometric at 400 nm
Total assay time:              ~ 30 min
Detection limit:                 0.05 U/mL
Application examples:
Cereal flours, fermentation broths and other materials
Method recognition:
AOAC (Method 2002.01), AACC (Method 22-02.01), ICC
(Standard No. 303), RACI (Standard Method) and CCFRA
(Flour Testing Working Group Method 0018)

Data Sheet: