1,2-α-L-Fucosidase (microbial)

Product code:

High purity recombinant 1,2-α-L-Fucosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.63
CAZy Family: GH95
CAS: 37288-45-2

1,2-alpha-L-fucosidase; 2-alpha-L-fucopyranosyl-beta-D-galactoside fucohydrolase

Recombinant. From a microbial source.
In solution (Tris.HCl/NaCl/EDTA).
Supplied at ~ 12 U/mL.

Specific activity:
~ 160 U/mg (37oC, pH 6.5 on 2’-fucosyllactose)

Stability:  > 3 years at 4oC.

Quantity:

Description

DESCRIPTION

1,2-α-L-Fucosidase (microbial)

EC 3.2.1.63
CAZy Family: GH95
CAS: 37288-45-2

Synonyms:
1,2-alpha-L-fucosidase; 2-alpha-L-fucopyranosyl-beta-D-galactoside fucohydrolase

Form:
In solution (Tris.HCl/NaCl/EDTA).

Stability:
> 3 years at 4oC.

Specific activity:
~ 160 U/mg (37oC, pH 6.5 on 2’-fucosyllactose).

Unit definition:
One Unit of α-1,2-L-fucosidase activity is defined as the amount of enzyme required to release one μmol of α-L-fucose per minute from 2’-fucosyllactose (2 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 37oC.

Specificity:
Highly specific for non-reducing terminal L-fucose residues linked to D-galactose residues by a 1,2-α-linkage. Does not hydrolyse p-nitrophenyl-α-L-fucopyranoside.

Applications:
For use in glycobiology research.