1-Kestopentaose

Product code: #KTW-1234

The L-Arabinose/D-Galactose test kit is a simple, reliable and accurate UV method for the measurement and analysis of L-arabinose and/or D-galactose in various materials including foods, feeds, beverages and plant products.

 

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Description

UV-method for the determination of L-Arabinose and D-Galactose in hydrolysed plant products Principle:

(galactose mutarotase) (1) α-L-Arabinose / α-D-galactose ↔ β-L-arabinose / β-D-galactose (β-galactose dehydrogenase) (2) β-L-Arabinose + NAD+ → L-arabinonic acid + NADH + H+ (β-galactose dehydrogenase) (3) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

 

Kit size:

* 115 assays (manual) / 1150 (microplate) / 1150 (auto-analyser)

* The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).

 

Method:

Spectrophotometric at 340 nm

 

Reaction time:

~ 12 min (L-arabinose) ~ 6 min (D-galactose)

 

Detection limit:

0.58 mg/L (L-arabinose) 0.69 mg/L (D-galactose)

 

Application examples:

Analysis of hydrolysates of oligo- and polysaccharides (e.g. arabinan, arabinoxylan, galactan, arabinogalactan), milk, dairy products, foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), food additives (e.g. sweeteners), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.)

 

Method recognition:

Novel method